A company wants to use the sober genes editing tool CRISPR to remove the major allergen from talk


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About 15% sober the human population is concerned by an allergy to domestic talk, and talk allergy awareness is associated with asthma. As current treatments remain limited in terms of their effectiveness, researchers from the biotechnology company InBio have used the CRISPR-Cas9 genome-editing method by targeting the tiny protein Fel g 1 (conversation’s major allergy). The results indicate that it is a practical tool to suppress Fel n 1 in the cells of flins, which could reduce the symptoms of people allergic to talks. However, it will take a few years to apply the method to our domestic talks.

The domestic talk (Felis catus) is the most common source of allergen inhalants derivatives sober mammals, result in sober the protein Fel chemical 1 secreted by the salivary and cutaneous glands sober all the talks. However, a study by 2019 suggests that the amounts of Fel d 1 secreted in saliva vary according to the discussion, without being able to confirm that certain contests are less likely to trigger allergy symptoms.

One thing is certain, more sober 90% of people allergic to talk present immunoglobulins E (IgE), ie antibodies against the primary allergen of talk. The objectives of the new US study were to identify sustain regions of Fel d 1 genes, and to delete certain Fel d 1 genes from feline cells using the CRISPR-Cas9 method (discovered in 2012). Specifically, the Fel d 1 protein is made up of two different subunits, and two genes (called CH1 and CH2) encode put for each subunit.

Researchers extracted sober DNA sober 50 domestic talks, in order to sequence the genes coding for CH1 and CH2. This allowed them to identify multiple sustained coding regions in the CH1 and CH2 genes, suitable for CRISPR genomic editing. genes sober Fel deb 1 tea raised more sober 25 unique amino acid alternatives of frequencies ranging from sober 2 92%, which gave rise to low polymorphisms Fel m 1 (several different configuration settings) with 50 99% ID

polymorphism fel d 1 cat genes

Analyse de Fel d 1 : structures en ruban (en haut) et en surface (en bas) avec les mutations de la chane 1 et de la chane 2 indiques en noir et violet fonc, respectivement. Brackett et al, 2022

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Analysis sober Fel g 1: buildings sober ribbon (sober high) and sober surface area (sober low) with mutations sober una chane 1 and sober una chain 2 indicates sober black and dark purple, respectively. Brackett et al, 20495

Genetic differences between domestic talkers and other species of talkers20495

They also found many differences between the CH1 and CH2 gene sequences of domestic talkers and those of eight other talker species such as lions, tigers, cougars and fishing talkers. These results imply that allergy may be non-essential for the talks, given the obvious lack of evolutionary preservation, write the authors of the article published in The CRISPR Log.

Our data indicates that Fel n 1 is once a rational and viable candidate for a deletion of the gene, which could be profoundly beneficial to people with allergies to sober talks eliminating the primary allergy to the supply, they add . Using the CRISPR method, they deleted either the CH1 gene or the CH2 gene from lifestyle conversational cells. We still have to try the method with the two genes at the same temperatures, in order to verify that the Fel d 1 home protein is not produced. According to the director of the CRISPR put talks team at InBio, the delay in applying the method to domestic talks should take several years.

Supply: The CRISPR Newspaper